| dc:title | Three-Dimensional-Printed Bone Grafts for Simultaneous Bone and Cartilage Regeneration: A Promising Approach to Osteochondral Tissue Engineering |
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| dc:creator | Paraš, Smiljana |
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| dc:creator | Petrović, Božana |
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| dc:creator | Mitić, Dijana |
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| dc:creator | Lazarević, Miloš |
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| dc:creator | Popović Bajić, Marijana |
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| dc:creator | Živković, Marija |
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| dc:creator | Mićić, Milutin |
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| dc:creator | Biočanin, Vladimir |
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| dc:creator | Živković, Slaviljub |
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| dc:creator | Jokanović, Vukoman |
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| dc:subject | osteochondral tissue engineering |
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| dc:subject | hydroxyapatite nanoparticles |
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| dc:subject | PLGA |
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| dc:subject | stem cells from apical papilla |
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| dc:subject | osteogenic differentiation |
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| dc:subject | chondrogenic differentiation |
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| dc:subject | cartilage regeneration |
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| dc:subject | bone regeneration |
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| dc:subject | segmental osteotomy |
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| dc:subject | femoral condyle |
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| dc:subject | hydroxyethyl cellulose |
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| dc:subject | sodium hyaluronate |
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| dc:subject | chondroitin sulfate |
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| dc:subject | biocompatibility |
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| dc:subject | vascularization |
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| dc:subject | graft resorption |
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| dc:description | Background/Objectives: A novel 3D-printed, bioresorbable bone graft, made of nanohydroxyapatite (nHAP) covered by poly(lactide-co-glycolide) (PLGA), showed strongly expressed osteoinductive properties in our previous investigations. The current study examines its application in dual regeneration of bone and cartilage by its combining with nHAP gel obtained by nHAP enrichment with hydroxyethyl cellulose, sodium hyaluronate, and chondroitin sulfate. Methods: In the in vitro part of the study, mitochondral activity and osteogenic and chondrogenic differentiation of stem cells derived from apical papilla (SCAPs) in the presence of nHAP gel were investigated. For in vivo part of the study, three rabbits underwent segmental osteotomies of lateral condyle of the femur, and defects were filled by 3D-printed grafts customized to the defect geometry. Results: In vitro study revealed that nHAP gel displayed significant biocompatibility, substantially increasing mitochondrial activity and facilitating osteogenic and chondrogenic differentiation of SCAPs. For in vivo part of the study, after a 12-week healing period, partial resorption of the graft was observed, and lamellar bone tissue with Haversian systems was detected. Histological and stereological evaluations of the implanted grafts indicated successful bone regeneration, marked by the infiltration of new bone and cartilaginous tissue into the graft. The existence of osteocytes and increased vascularization indicated active osteogenesis. The hyaline cartilage near the graft showed numerous new chondrocytes and the significant layer of newly formed cartilage. Conclusions: This study demonstrated that tailored 3D-printed bone grafts could efficiently promote the healing of substantial bone defects and formation of new cartilage without requiring supplementary biological factors, offering a feasible alternative for clinical bone repair applications. |
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| dc:publisher | Multidisciplinary Digital Publishing Institute |
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| dc:date | 2025 |
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| dc:type | info:eu-repo/semantics/article |
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| dc:type | info:eu-repo/semantics/publishedVersion |
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| dc:identifier | https://stomatoloskifakultet.rs/nauka/publikacija/927 |
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| dc:identifier | DOI 10.3390/pharmaceutics17040489 |
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| dc:identifier | ISSN 1999-4923 |
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| dc:source | Pharmaceutics 17(4): 489 |
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| dc:language | en |
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| dc:rights | info:eu-repo/semantics/closedAccess |
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| dc:rights | Sva prava zadržana |
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